在WB试验中中，最重要和最不可或缺的对照就是内参照（Loading Control，Internal Control）。内参照的使用有两个方面的作用： 是检测整个WB实验过程及体系是否正常工作，如果一个实验连内参照都出不了阳性结果的话，那么这个体系就是存在问题的了 。 半定量的标准：内参一般选择的管家基因编码表达的蛋白，在很多组织和细胞中中都是稳定表达的，因此可以作为检测靶蛋白表达量的标准。
Rabbit polyclonal to beta actin (ab8227) at 1/5000. Lane 1: HeLa whole cell extract (20ug); Lane 2: yeast cell extract (20ug) Lane 3: mouse brain tissue lysate (20ug). Developed using Goat anti-rabbit IgG (HRP) (ab6721) at 1/2000. Don’t be a cowboy, use a Loading Control! WB常用内参及其技术参数： 内参名称 | 分子量大小 | 适用范围 | beta-actin | 43kDa | 胞浆和全细胞 | GAPDH | 30-40kDa | 胞浆和全细胞 | Tubulin | 55kDa | 胞浆和全细胞 | VCDA1/Porin | 31kDa | 线粒体 | COXIV | 16kDa | 线粒体 | TBP | 38kDa | 细胞核 |
Abcam作为全球最大抗体供应商，拥有非常齐全的Loading Control，绝对能满足您WB的需求！ 目录号 | 产品描述 | 规格 | 价格 | ab6276 | beta Actin antibody [AC-15]-loading control | 100 ul | 3968 | ab8226 | beta Actin antibody [mAbcam 8226] - Loading Control | 100ug | 3968 | ab8226 | beta Actin antibody [mAbcam 8226] - Loading Control | 25ug | 1380 | ab8227 | beta Actin antibody - Loading Control | 50ul | 3968 | ab13822 | beta Actin antibody - Loading Control | 100ug | 3450 | ab9484 | GAPDH antibody [mAbcam 9484] - Loading Control | 200ug | 3968 | ab9485 | GAPDH antibody - Loading Control | 100ug | 3795 | ab9485 | GAPDH antibody - Loading Control | 25ug | 1553 | ab15859 | VDAC1 / Porin antibody - Mitochondrial Loading Control | 100ug | 3795 | ab15859 | VDAC1 / Porin antibody - Mitochondrial Loading Control | 25ug | 1380 | ab14734 | VDAC1 / Porin antibody [20B12] – Mitochondrial Loading Control | 100ug | 3450 | ab6046 | beta Tubulin antibody – Loading Control | 100ug | 3795 | ab6046 | beta Tubulin antibody – Loading Control | 25ug | 1380 | ab21057 | beta Tubulin antibody - Loading Control | 100ug | 3450 | ab4074 | alpha Tubulin antibody - Loading Control | 100ug | 3795 | ab4074 | alpha Tubulin antibody - Loading Control | 25ug | 1380 | ab7291 | alpha Tubulin antibody [DM1A] - Loading Control | 100ug | 3795 | ab7291 | alpha Tubulin antibody [DM1A] - Loading Control | 25ug | 1380 | ab14744 | COX IV antibody [20E8] - Loading Control | 100ug | 3795 | ab16056 | COX IV antibody - Mitochondrial Loading Control | 100ug | 3795 | ab16056 | COX IV antibody - Mitochondrial Loading Control | 25ug | 1380 | ab33985 | COX IV antibody - Mitochondrial Loading Control | 100ug | 3968 | ab818 | TBP antibody [1TBP18] - Nuclear Loading Control | 100ug | 3968 | ab62126 | TBP antibody [mAbcam 62126] - Nuclear Loading Control and ChIP Grade | 100ug | 3450 |
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